蚊子通过轮胎搭便车
二手轮胎的全球贸易是负责的主要因素用于介绍Aedesinvasive mosquitoes (AIMs) that are major disease vectors (e.g. dengue, Zika, chikungunya and yellow fever). As the eggs of埃及埃及andAe。白化病可以承受几个月的干燥，它们可以在二手轮胎中搭便车沿全球搭m脚。A classic example of how easily these tropical and subtropical species can enter new territories is the 4600-mile journeyAe。aegyptitook fromFlorida to The Netherlands via a shipment of used tyres。
With climate change, AIMs can find themselves in regions more climatically suitable than it would have been previously possible, bringing with them the threat of disease.艾德（Aedes botictus）is now one of the most地球上的侵入性动物物种。
目前用于使用的监视方法adult traps and larval dippingi.e. catching adult mosquitoes and the larvae of a breeding population. The ideal scenario would be to catch AIMs eggs coming into a new territory at the point of entry before adults emerge, females blood feed (which itself runs a risk due to登革热的跨动力传播），，，，mate and potentially establish a breeding population. Currently, no tested method exists to screen imported used tyres for eggs in diapause, the life stage most at risk from accidental introduction.
在一杯茶上进行头脑风暴
After a day carrying out AIM surveillance at Liverpool Port using adult traps, over a cup of tea we came up with the idea of using adhesive tape to screen used tyres. In the lab we tested four inexpensive, readily available tapes to see how effective they were in picking upAe。aegypti二手车轮胎的鸡蛋。我们测试了胶带，包装胶带，透明的胶带和身体胶带（对后者进行了测试珍妮弗·洛佩兹（Jennifer Lopez）穿着范思哲（Versace）连衣裙！）。
成功接送
所有磁带都成功地捡起了Ae。aegyptieggs, whilst the efficiency was less on dirty tyres compared to a clean one, pick up of eggs was still possible. Thesuccesswe saw in the lab was three-fold.
首先，只需通过视觉检查胶带就可以怀疑轮胎上的鸡蛋的存在，就意味着可以持有货物，直到确认识别为止。如果不可能的话，货物至少可以追踪到其最终目的地，然后可以对其进行轮胎杀死鸡蛋。这具有主要优势，即能够在卵孵化之前杀死鸡蛋，后来又出现了成年人。如果轮胎测试目标卵呈阳性，则可以识别出它们起源的高风险区域，并采取适当的措施。
其次，粘合剂不会干扰胶带的DNA扩增，这些卵子可用于物种特异性鉴定和筛选病毒DNA的存在。DNA扩增也可以帮助人群遗传研究。10天后，DNA仍然可以放大，这将为卫生当局提供足够的时间发布涉嫌在合适的实验室进行测试的磁带。
第三，我们能够将带胶带的鸡蛋延到成人身上。实验室中蚊子种群定居的能力允许进一步研究（例如，杀虫剂的耐药性分析可以为适当的控制措施提供信息）。
这个新的监测方法可能成为一个标准rdised tool with global applicability. In turn, it could lead to increased predictability of introductions and improve screening methods at high risk entry points. We have recommended a procedure for screening used tyres with adhesive tape and the potential pathways that can be taken. Who would have thought that humble tape could be a potential weapon in the fight against arboviral diseases thatthreaten 50% of the world’s population呢
从实验室到现场
我们的下一步是测试现场中的方法。海斯汉姆（Heysham）和格拉森（Glasson）码头（英国）的港口卫生官员用胶带胶带录制了轮胎，并成功地将磁带送到了我们对鸡蛋进行筛选的帖子中。我们现在正在寻求扩大监视，以包括更多的高风险区域，并为在AIM监视中工作的人开发一个简单的用户友好协议，而使用的轮胎构成了威胁。
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