您能给我们一些有关您的项目的背景信息，以及为什么它很重要？
My name is Julie Gervais and I am a third year PhD student in INRA BIOGER (France) working on a fungal pathogen of oilseed rape,Leptosphaeria maculans, which is responsible for the stem canker disease. This fungus has two colonization stages of the plant. During the first stage, the fungus infects leaves and cotyledons (the first leaves to appear from a germinating seed). Once in the leaves, the fungus has a short biotrophic stage of 10 days and then switches to necrotrophy.
Following this primary leaf infection,L. maculansgrows inside the stem tissues during a long endophytic systemic colonization. This colonization is completely symptomless and may last up to 9 months. I am aiming to gain a better understanding of how the fungus can grow inside the oilseed rape stem for several months without causing any symptoms. I am particularly focused on the identification of new effectors, secreted proteins, produced by the fungus enabling it to develop itself efficiently into the plant.
Could you briefly describe your research and your findings?
我特别专注于鉴定新效应子，即由真菌产生的分泌蛋白，使其能够有效地发展到植物中。
Bytranscriptomic analysis, I identified ‘late’ effector candidates, under-expressed in the early colonization stage and over-expressed in the infected stems. My analysis revealed a link between the regulation of expression of effectors and their genomic location: the ‘late’ effector candidates, putatively involved in systemic colonization, are located in gene-rich genomic regions, whereas the ‘early’ effector genes, over-expressed in the early colonization stage, are located in gene-poor regions of the genome. These results were recently published in an article ofMolecular Plant Pathology.
我还试图确认六个后期效应候选者的效应子的作用：我正在测量这些基因对茎内真菌生长的沉默的影响。初步结果表明，这些候选者之一的沉默会导致茎上较小的坏死。
Another aim of my thesis is to identify new resistances to controlL. maculans.The identification of new effector genes would contribute to the identification of new resistance genes specific to these effectors.
是什么让您与真菌一起工作？您最喜欢研究什么？
During my studies, I became more interested in the understanding of interactions between plants and micro-organisms, so I decided to pursue this interest in my thesis onL. maculansand its host, oilseed rape. I especially enjoy trying to dissect the network of interactions between the two organisms and to be able to apply such findings in the effective control of plant diseases.
What advice would you give young scientists starting out in research?
I would advise young scientists to stay focused on what they are interested in and to always take pleasure in what they do. Science is fun!
Acknowledgements
我能够参加真菌遗传学会议，这要归功于“AcadémieD'Acriculture（Grant Jean＆Marie＆Marie＆Marie＆Marie dufrenoy）的旅行资助”和美国遗传学协会的旅行。
Enjoyed reading this blog? Read our other Fungal Genetics Conference Q&As here:
Comments